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<span style="font-size:12.0pt; font-family:"Times New Roman",serif"><img width="600" height="171" id="Picture_x0020_3" src="cid:image005.jpg@01D845D5.17B10F60" alt="Dissertation Defense Announcement at the Cullen College of Engineering" style="width:6.25in; height:1.7812in"></span><span style="font-size:12.0pt; font-family:"Times New Roman",serif"></span></p>
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<b><span style="font-size:16.0pt; font-family:"Times New Roman",serif; color:red; text-transform:uppercase">Detection of Biomolecules using Novel Approaches</span></b></p>
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<b><span style="font-size:13.5pt; font-family:"Times New Roman",serif">Atul Goyal</span></b><span style="font-size:13.5pt; font-family:"Times New Roman",serif"></span></p>
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<span style="font-family:"Times New Roman",serif">August 2, 2022; 10:30 AM (CST)</span></p>
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<span style="font-family:"Times New Roman",serif"><br>
<b>Room:</b> Chemical Engineering Conference Room</span></p>
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<b><span style="font-size:12.0pt; font-family:"Times New Roman",serif; color:black">Zoom Meeting ID</span></b><span style="font-size:12.0pt; font-family:"Times New Roman",serif; color:black">: </span></p>
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<span style="font-size:10.5pt; font-family:"Times New Roman",serif; color:#252424"> </span><a href="https://urldefense.com/v3/__https:/us02web.zoom.us/j/85412637467pwd=Q1dHOFBnY1RXYXhHUmw2dmkvaEZSUT09__;!!LkSTlj0I!GU4M33VF95bEGI4eSpyeQQyVGrfWNAkF_Oux654niyANqAxE-YWZpH7aV3nuIEphzTU6P11L54KcDmpoPZnWmuOWS24$">https://us02web.zoom.us/j/85412637467pwd=Q1dHOFBnY1RXYXhHUmw2dmkvaEZSUT09</a><span style="font-family:"Times New Roman",serif"></span></p>
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<b><span style="font-family:"Times New Roman",serif">Committee Chair:</span></b><span style="font-family:"Times New Roman",serif"><br>
Richard Willson, Ph.D.</span></p>
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<b><span style="font-family:"Times New Roman",serif">Committee Members:</span></b><span style="font-size:10.0pt; font-family:"Times New Roman",serif"><br>
Navin Varadarajan, Ph.D. <b>| </b>Gul Zerze, Ph.D. <b>|</b> Jiming Bao, Ph.D. <b>
|</b> Paul Ruchhoeft, PhD.</span><span style="font-size:10.5pt; font-family:"Times New Roman",serif"></span></p>
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<b><span style="font-size:12.0pt; font-family:"Times New Roman",serif; color:#C8102E">Abstract</span></b><span style="font-size:12.0pt; font-family:"Times New Roman",serif; color:#C8102E"></span></p>
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<span style="font-family:"Times New Roman",serif; color:black">In the manufacture of therapeutic monoclonal antibodies (mAbs), the clarified cell culture fluid is typically loaded onto an initial protein A affinity capture column. Imperfect mass transfer and
loading to maximum capacity can risk antibody breakthrough and loss of valuable product, but conservative underloading wastes expensive protein A resin. In addition, the effects of column fouling and ligand degradation require the frequent optimization of
IgG loading to avoid wastage. Therefore, continuous real-time monitoring of IgG flowthrough is of great interest. We previously developed a fluorescence-based monitoring technology that allows mix-and-read mAb detection in cell culture fluid. Here we report
the use of reporters immobilized on CNBr-activated Sepharose 4B resin for continuous detection of IgG in column breakthrough. The column effluent is continuously contacted with immobilized fluorescein-labeled Fc-binding ligands to produce an immediately detectable
change in fluorescence intensity. The technology allows rapid and reliable monitoring of IgG in a flowing stream of clarified cell culture fluid emerging from a protein A column, without prior sample preparation. We observed a significant change in fluorescence
intensity at 0.5 g/L human IgG, sufficient to detect a 5% breakthrough of a 10 g/L load, within 2 minutes at a flow rate of 0.5 mL/min.</span></p>
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<span style="font-family:"Times New Roman",serif; color:black">In another study, we investigated the affinity capture of nanoparticles using membrane chromatography. A major cause of the continuous wide spread of COVID-19 is the lack of a fast, cheap, and reliable
diagnostic technique. For the most reliable COVID-19 result, the PCR testing still has to be performed by medical specialists at properly-equipped sites, and both patients and health authorities sometimes have to wait for days to get the results, significantly
hampering efforts to control and minimize the spread of the virus. This research aimed to develop an ultra-sensitive detection method for the novel coronavirus and other analytes that is inexpensive, portable, and does not require PCR amplification or its
associated expensive equipment. We used micro and nanoscale holes through which light can pass and which can be occluded by single nanoparticles as small as 110 nm in diameter. While chemistry for the successful affinity capture of antibody conjugated nanoparticles
to effectively deliver to nanoholes for detection was tested, it continues to be developed..</span><span style="font-size:12.0pt; line-height:150%; font-family:"Times New Roman",serif">
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