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<span style="font-family: Calibri, sans-serif; font-size: 11pt;">The UH Sequencing and Gene Editing Core has a Request for Proposals that is open to UH investigators only. Eligible engineering faculty are encouraged to apply. The proposal submission deadline
is Jan. 10, 2020. Please see attached and below for details. </span><br>
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Kathy</p>
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<span style="font-size:14.0pt; font-family:"Arial",sans-serif">---------------</span></p>
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<b><u><span style="font-size:14.0pt; font-family:"Arial",sans-serif">REQUEST FOR PROPOSALS</span></u></b></p>
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<b><span style="font-size:14.0pt; font-family:"Arial",sans-serif">Sequencing and Gene Editing Core - Single cell RNA Sequencing (scRNA-Seq) Award</span></b></p>
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<b><u><span style="font-size:10.5pt; font-family:"Arial",sans-serif"><span style="text-decoration:none"> </span></span></u></b></p>
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<b><u><span style="font-size:10.5pt; font-family:"Arial",sans-serif">BACKGROUND</span></u></b></p>
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<b><span style="font-size:10.5pt; font-family:"Arial",sans-serif"> </span></b></p>
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<span style="font-size:10.5pt; font-family:"Arial",sans-serif">Some of the most significant insights into the complex etiology and diversity of various diseases and dysfunctions have been discovered through genomic profiling and accelerated via next-generation
sequencing. These findings have been magnified by the ability to probe at single-cell resolution. The 10x Genomics single cell technology can analyze up to 10,000 single cells alongside Illumina’s next-generation sequencing platform. The UH Sequencing and
Gene Editing Core supports UH researchers by providing access to this single-cell gene expression technology, analysis, and integration.
</span></p>
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<span style="font-size:10.5pt; font-family:"Arial",sans-serif"> </span></p>
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<span style="font-size:10.5pt; font-family:"Arial",sans-serif">This Single-Cell RNA Sequencing Award covers library preparation and bioinformatics support for up to two (2), two (2) sample pilot projects using the single cell gene expression sequencing application
(</span><a href="https://www.10xgenomics.com/solutions/single-cell/"><span style="font-size:10.5pt; font-family:"Arial",sans-serif">https://www.10xgenomics.com/solutions/single-cell/</span></a><span style="font-size:10.5pt; font-family:"Arial",sans-serif">)
from 10x Genomics.</span></p>
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<b><u><span style="font-size:10.5pt; font-family:"Arial",sans-serif">PURPOSE</span></u></b></p>
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<b><span style="font-size:10.5pt; font-family:"Arial",sans-serif"> </span></b></p>
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<span style="font-size:10.5pt; font-family:"Arial",sans-serif">The goals of this funding opportunity are three-fold:
</span></p>
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<span style="font-size:10.5pt; font-family:"Arial",sans-serif"> </span></p>
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<span style="font-size:10.5pt; font-family:"Arial",sans-serif"><span style="">(1)<span style="font:7.0pt "Times New Roman"">
</span></span></span><span style="font-size:10.5pt; font-family:"Arial",sans-serif">support investigators’ use of scRNA-seq to acquire preliminary data for external funding, test feasibility of scRNA-seq as an approach, and/or validate data needed for publication;
</span></p>
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<span style="font-size:10.5pt; font-family:"Arial",sans-serif"> </span></p>
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<span style="font-size:10.5pt; font-family:"Arial",sans-serif"><span style="">(2)<span style="font:7.0pt "Times New Roman"">
</span></span></span><span style="font-size:10.5pt; font-family:"Arial",sans-serif">encourage the use of scRNA-seq by investigators who are new to the technology; and</span></p>
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<span style="font-size:10.5pt; font-family:"Arial",sans-serif"> </span></p>
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</span></span></span><span style="font-size:10.5pt; font-family:"Arial",sans-serif">promote a collaborative environment between the UH Sequencing and Gene Editing Core and UH Researchers.</span></p>
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<b><u><span style="font-size:10.5pt; font-family:"Arial",sans-serif"><span style="text-decoration:none"> </span></span></u></b></p>
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<b><u><span style="font-size:10.5pt; font-family:"Arial",sans-serif">FUNDING</span></u></b></p>
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<b><span style="font-size:10.5pt; font-family:"Arial",sans-serif"> </span></b></p>
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<span style="font-size:10.5pt; font-family:"Arial",sans-serif">The UH Sequencing and Gene Editing Core will provide the following to the selected winners:</span></p>
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</span></span></span><span style="font-size:10.5pt; font-family:"Arial",sans-serif">Project Consultation</span></p>
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</span></span></span><span style="font-size:10.5pt; font-family:"Arial",sans-serif">Sample Quantification/QC</span></p>
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</span></span></span><span style="font-size:10.5pt; font-family:"Arial",sans-serif">Single Cell capture and Library Preparation
</span></p>
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</span></span></span><span style="font-size:10.5pt; font-family:"Arial",sans-serif">Next-generation Sequencing
</span></p>
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</span></span></span><span style="font-size:10.5pt; font-family:"Arial",sans-serif">Bioinformatics Support</span></p>
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</span></span></span><span style="font-size:10.5pt; font-family:"Arial",sans-serif">Invitation to present at the following UH Sequencing and Gene Editing Core seminar.</span></p>
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<span style="font-size:10.5pt; font-family:"Arial",sans-serif"> </span></p>
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<b><u><span style="font-size:10.5pt; font-family:"Arial",sans-serif">ELIGIBILITY</span></u></b></p>
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<span style="font-size:10.5pt; font-family:"Arial",sans-serif"> </span></p>
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<span style="font-size:10.5pt; font-family:"Arial",sans-serif">ONLY University of Houston investigators are eligible to apply. Those that attended the UH Sequencing and Gene Editing Core seminar (11/21/19) will receive additional consideration. Laboratories
can submit more than one (1) proposal; however, only one (1) proposal within a laboratory can be selected as a winner.
</span></p>
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<span style="font-size:10.5pt; font-family:"Arial",sans-serif"> </span></p>
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<b><u><span style="font-size:10.5pt; font-family:"Arial",sans-serif">PROPOSAL REQUIREMENTS
</span></u></b></p>
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<span style="font-size:10.5pt; font-family:"Arial",sans-serif"> </span></p>
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</span></span></span><span style="font-size:10.5pt; font-family:"Arial",sans-serif">Proposal abstract within a max of 300 words.</span></p>
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</span></span></span><span style="font-size:10.5pt; font-family:"Arial",sans-serif">Abstract should include:
</span></p>
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</span></span></span><span style="font-size:10.5pt; font-family:"Arial",sans-serif">Background/Rationale</span></p>
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</span></span></span><span style="font-size:10.5pt; font-family:"Arial",sans-serif">scRNA-seq Approach using 10X Genomics gene expression platform.</span></p>
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<a href="https://www.10xgenomics.com/solutions/single-cell/"><span style="font-size:10.5pt; font-family:"Arial",sans-serif">https://www.10xgenomics.com/solutions/single-cell/</span></a><span style="font-size:10.5pt; font-family:"Arial",sans-serif"></span></p>
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</span></span></span><span style="font-size:10.5pt; font-family:"Arial",sans-serif">scRNA-seq Impact</span></p>
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</span></span></span><span style="font-size:10.5pt; font-family:"Arial",sans-serif">Overall Research Significance</span></p>
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</span></span></span><span style="font-size:10.5pt; font-family:"Arial",sans-serif">Format:
</span></p>
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</span></span></span><span style="font-size:10.5pt; font-family:"Arial",sans-serif">US Letter size layout paper (8.5”x11”)
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</span></span></span><span style="font-size:10.5pt; font-family:"Arial",sans-serif">1-inch margins on all sides
</span></p>
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<span style="font-size:10.5pt; font-family:"Courier New""><span style="">o<span style="font:7.0pt "Times New Roman"">
</span></span></span><span style="font-size:10.5pt; font-family:"Arial",sans-serif">Arial font size 11 pt.</span></p>
<p class="MsoListParagraphCxSpMiddle" style="margin: 0in 0in 0.0001pt 0.5in; line-height: 105%; font-size: 11pt; font-family: Calibri, sans-serif;margin-right:0in; margin-bottom:0in; margin-left:1.0in; margin-bottom:.0001pt; text-align:justify; text-indent:-.25in; line-height:normal; background:white; vertical-align:baseline">
<span style="font-size:10.5pt; font-family:"Courier New""><span style="">o<span style="font:7.0pt "Times New Roman"">
</span></span></span><span style="font-size:10.5pt; font-family:"Arial",sans-serif">1.5 line spacing
</span></p>
<p class="MsoListParagraphCxSpMiddle" style="margin: 0in 0in 0.0001pt 0.5in; line-height: 105%; font-size: 11pt; font-family: Calibri, sans-serif;margin-right:0in; margin-bottom:0in; margin-left:1.0in; margin-bottom:.0001pt; text-align:justify; text-indent:-.25in; line-height:normal; background:white; vertical-align:baseline">
<span style="font-size:10.5pt; font-family:"Courier New""><span style="">o<span style="font:7.0pt "Times New Roman"">
</span></span></span><span style="font-size:10.5pt; font-family:"Arial",sans-serif">One (1) figure/table can be included within the proposal. The caption text must be legible (Arial; 8 pt. min.) and will not be counted towards 300-word max.</span></p>
<p class="MsoListParagraphCxSpLast" style="margin: 0in 0in 8pt 0.5in; line-height: 105%; font-size: 11pt; font-family: Calibri, sans-serif;margin-right:0in; margin-bottom:0in; margin-left:1.0in; margin-bottom:.0001pt; text-align:justify; text-indent:-.25in; line-height:normal; background:white; vertical-align:baseline">
<span style="font-size:10.5pt; font-family:"Courier New""><span style="">o<span style="font:7.0pt "Times New Roman"">
</span></span></span><span style="font-size:10.5pt; font-family:"Arial",sans-serif">Applications that do not follow the formatting requirements will not be reviewed.</span></p>
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<span style="font-size:10.5pt; font-family:"Arial",sans-serif"> </span></p>
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<b><u><span style="font-size:10.5pt; font-family:"Arial",sans-serif">SUBMISSION REQUIREMENTS AND TIMELINE</span></u></b></p>
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<span style="font-size:10.5pt; font-family:"Arial",sans-serif"> </span></p>
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<span style="font-size:10.5pt; font-family:"Arial",sans-serif">Submit the completed application (.pdf only) to
</span><a href="mailto:uhsequencing@gmail.com"><span style="font-size:10.5pt; font-family:"Arial",sans-serif">uhsequencing@gmail.com</span></a><span style="font-size:10.5pt; font-family:"Arial",sans-serif">.</span></p>
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<span style="font-size:10.5pt; font-family:"Arial",sans-serif"> </span></p>
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<span style="font-size:10.5pt; font-family:"Arial",sans-serif">Submit document with the following Subject line and filename format:</span></p>
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<span style="font-size:10.5pt; font-family:"Arial",sans-serif"> </span></p>
<p class="MsoNormal" style="margin: 0in 0in 8pt; line-height: 105%; font-size: 11pt; font-family: Calibri, sans-serif;margin-bottom:0in; margin-bottom:.0001pt; text-align:justify; line-height:normal; background:white; vertical-align:baseline">
<span style="font-size:10.5pt; font-family:"Arial",sans-serif">“SingleCell_LastName_FirstName”</span></p>
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<b><span style="font-size:10.5pt; font-family:"Arial",sans-serif"> </span></b></p>
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<i><span style="font-size:10.5pt; font-family:"Arial",sans-serif">Ex) SingleCell_Doe_John</span></i></p>
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<span style="font-size:10.5pt; font-family:"Arial",sans-serif"> </span></p>
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<b><span style="font-size:10.5pt; font-family:"Arial",sans-serif">Submission Opens:</span></b><span style="font-size:10.5pt; font-family:"Arial",sans-serif"> Thursday, November 21, 2019</span></p>
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<span style="font-size:10.5pt; font-family:"Arial",sans-serif"> </span></p>
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<b><span style="font-size:10.5pt; font-family:"Arial",sans-serif">Proposal Submission Deadline</span></b><span style="font-size:10.5pt; font-family:"Arial",sans-serif">: Friday, January 10, 2020 5:00 PM (CST)</span></p>
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<span style="font-size:10.5pt; font-family:"Arial",sans-serif"> </span></p>
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<b><span style="font-size:10.5pt; font-family:"Arial",sans-serif">Winner(s) Announced by:</span></b><span style="font-size:10.5pt; font-family:"Arial",sans-serif"> Monday, February 10, 2020</span></p>
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<span style="font-size:10.5pt; font-family:"Arial",sans-serif"> </span></p>
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<b><span style="font-size:10.5pt; font-family:"Arial",sans-serif">Start of Projects</span></b><span style="font-size:10.5pt; font-family:"Arial",sans-serif">: latest 6 months after selection notification.</span></p>
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<span style="font-size:10.5pt; font-family:"Arial",sans-serif"> </span></p>
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<b><u><span style="font-size:10.5pt; font-family:"Arial",sans-serif">CONTACT INFORMATION</span></u></b></p>
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<span style="font-size:10.5pt; font-family:"Arial",sans-serif"> </span></p>
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<span style="font-size:10.5pt; font-family:"Arial",sans-serif">General questions related to the RFP/Submission should be directed to
</span><a href="mailto:kholloway3@uh.edu"><span style="font-size:10.5pt; font-family:"Arial",sans-serif">kholloway3@uh.edu</span></a><span style="font-size:10.5pt; font-family:"Arial",sans-serif"> or 713-743-9291 (SERC 4008 office).</span></p>
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<span style="font-size:10.5pt; font-family:"Arial",sans-serif"> </span></p>
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<b><u><span style="font-size:10.5pt; font-family:"Arial",sans-serif">WEBSITE</span></u></b></p>
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<b><u><span style="font-size:10.5pt; font-family:"Arial",sans-serif"><span style="text-decoration:none"> </span></span></u></b></p>
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<span style="font-family:"Arial",sans-serif"><a href="http://seqnedit.nsm.uh.edu/">http://seqnedit.nsm.uh.edu/</a></span></p>
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