<html><head>
<meta http-equiv="Content-Type" content="text/html; charset=Windows-1252"></head><body style="word-wrap: break-word; -webkit-nbsp-mode: space; -webkit-line-break: after-white-space; color: rgb(0, 0, 0); font-size: 14px; font-family: Calibri, sans-serif; "><div><br></div><span id="OLK_SRC_BODY_SECTION"><div xmlns:v="urn:schemas-microsoft-com:vml" xmlns:o="urn:schemas-microsoft-com:office:office" xmlns:w="urn:schemas-microsoft-com:office:word" xmlns:x="urn:schemas-microsoft-com:office:excel" xmlns:m="http://schemas.microsoft.com/office/2004/12/omml" xmlns="http://www.w3.org/TR/REC-html40"><div lang="EN-US" link="blue" vlink="purple"><div class="WordSection1"><p class="MsoNormal"><o:p> </o:p></p><p class="MsoNormal" align="center" style="mso-margin-top-alt:auto;margin-bottom:6.0pt;text-align:center;line-height:18.0pt"><a name="_Toc434675189"><b><span style="font-size: 14pt; font-family: 'Times New Roman', serif; color: black; ">MS DEFENSE STUDENT: </span></b></a><span style="font-size: 14pt; font-family: 'Times New Roman', serif; color: black; ">Rajat Ghosh</span><span style="font-family: 'Times New Roman', serif; color: black; "><o:p></o:p></span></p><p class="MsoNormal" align="center" style="mso-margin-top-alt:auto;margin-bottom:6.0pt;text-align:center;line-height:18.0pt"><b><span style="font-size: 14pt; font-family: 'Times New Roman', serif; color: black; ">DATE: </span></b><span style="font-size: 14pt; font-family: 'Times New Roman', serif; color: black; ">Monday, November 28, 2016</span><span style="font-family: 'Times New Roman', serif; color: black; "><o:p></o:p></span></p><p class="MsoNormal" align="center" style="mso-margin-top-alt:auto;margin-bottom:6.0pt;text-align:center;line-height:18.0pt"><b><span style="font-size: 14pt; font-family: 'Times New Roman', serif; color: black; ">TIME:
</span></b><span style="font-size: 14pt; font-family: 'Times New Roman', serif; color: black; ">10:00 AM</span><span style="font-family: 'Times New Roman', serif; color: black; "><o:p></o:p></span></p><p class="MsoNormal" align="center" style="mso-margin-top-alt:auto;margin-bottom:6.0pt;text-align:center;line-height:18.0pt"><b><span style="font-size: 14pt; font-family: 'Times New Roman', serif; color: black; ">PLACE:</span></b><span style="font-size: 14pt; font-family: 'Times New Roman', serif; color: black; "> Large Mechanical Engineering Conference Room</span><span style="font-family: 'Times New Roman', serif; color: black; "><o:p></o:p></span></p><p class="MsoNormal" align="center" style="mso-margin-top-alt:auto;margin-bottom:6.0pt;text-align:center;line-height:18.0pt"><b><span style="font-size: 14pt; font-family: 'Times New Roman', serif; color: black; ">DISSERTATION CHAIR: </span></b><span style="font-size: 14pt; font-family: 'Times New Roman', serif; color: black; ">Dr. Peter Vekilov</span><span style="font-family: 'Times New Roman', serif; color: black; "><o:p></o:p></span></p><div class="MsoNormal" align="center" style="margin-bottom:0in;margin-bottom:.0001pt;text-align:center;line-height:115%"><b><span style="font-size: 14pt; line-height: 115%; font-family: 'Times New Roman', serif; color: black; "><hr size="2" width="100%" align="center"></span></b></div><p class="MsoNormal" align="center" style="margin-bottom:0in;margin-bottom:.0001pt;text-align:center;line-height:150%"><b><span style="font-size: 14pt; line-height: 150%; font-family: 'Times New Roman', serif; color: black; ">TITLE:<o:p></o:p></span></b></p><p class="MsoNormal" align="center" style="margin-bottom:0in;margin-bottom:.0001pt;text-align:center;line-height:150%"><b><span style="font-size: 14pt; line-height: 150%; font-family: 'Times New Roman', serif; color: black; ">MESOSCOPIC PROTEIN-RICH PHASES IN RIBONUCLEASE A SOLUTION<o:p></o:p></span></b></p><p class="MsoNormal" style="text-align:justify;line-height:200%"><b><span style="font-size: 12pt; line-height: 200%; font-family: 'Times New Roman', serif; "><o:p> </o:p></span></b></p><p class="MsoNormal" style="text-align:justify;line-height:200%"><span style="font-size: 12pt; line-height: 200%; font-family: 'Times New Roman', serif; ">Dense liquid phases called clusters exist in protein solutions and act as precursors to crystal nucleation.
Clusters have a narrow size distribution and submicron size. These clusters are present in a minor fraction of total protein, not affecting the properties of solution, but are essential precursors for the nucleation of ordered species. The experiments here
are done with acidic protein solution of Ribonuclease A (RNase A). Here we studied the effect of ionic strength, urea and temperature with the help of Brownian microscopy (BM), static light scattering (SLS), and several biochemical assays. The cluster size
distribution was measured for three days with BM and revealed a consistently narrow size distribution through this time period, indicating stability of clusters. The clusters respond to the change in protein concentration, this change is exhibited as decrease
in cluster volume fraction as the protein concentration was increased indicating these clusters are not irreversible aggregates. The intermolecular forces were varied by changing the ionic strength with NaCl, (NH4)2SO4 and CH3COONa and quantified by measuring
second virial coefficient by SLS. The data revealed that intermolecular repulsion decreases the cluster size and cluster volume fraction. The hydrophobic interactions between the exposed hydrophobic residues due to partial unfolding and solvent was studied
by the addition of urea, a chaotropic agent that destroys the shell of structured water around protein molecules. Elman’s assay was used to quantify the free thiols and the degree of unfolding was studied using ANS and ThT assays. The unfolding of protein
by breaking disulphide bonds by heating showed an increase in cluster size and cluster volume fraction indicating the role of protein unfolding to aid the cluster formation. These experiments demonstrated the relevance of partial unfolding and intermolecular
interactions in formation of clusters.<o:p></o:p></span></p></div></div></div></span><style><!--
/* Font Definitions */
@font-face
{font-family:"Cambria Math";
panose-1:2 4 5 3 5 4 6 3 2 4;}
@font-face
{font-family:Calibri;
panose-1:2 15 5 2 2 2 4 3 2 4;}
@font-face
{font-family:Tahoma;
panose-1:2 11 6 4 3 5 4 4 2 4;}
@font-face
{font-family:"Century Gothic";
panose-1:2 11 5 2 2 2 2 2 2 4;}
/* Style Definitions */
p.MsoNormal, li.MsoNormal, div.MsoNormal
{margin-top:0in;
margin-right:0in;
margin-bottom:8.0pt;
margin-left:0in;
line-height:105%;
font-size:11.0pt;
font-family:"Calibri","sans-serif";}
a:link, span.MsoHyperlink
{mso-style-priority:99;
color:blue;
text-decoration:underline;}
a:visited, span.MsoHyperlinkFollowed
{mso-style-priority:99;
color:purple;
text-decoration:underline;}
p.MsoAcetate, li.MsoAcetate, div.MsoAcetate
{mso-style-priority:99;
mso-style-link:"Balloon Text Char";
margin:0in;
margin-bottom:.0001pt;
font-size:8.0pt;
font-family:"Tahoma","sans-serif";}
span.EmailStyle17
{mso-style-type:personal;
font-family:"Times New Roman","serif";
color:black;
font-weight:bold;}
span.EmailStyle18
{mso-style-type:personal-reply;
font-family:"Calibri","sans-serif";
color:#1F497D;}
span.BalloonTextChar
{mso-style-name:"Balloon Text Char";
mso-style-priority:99;
mso-style-link:"Balloon Text";
font-family:"Tahoma","sans-serif";}
.MsoChpDefault
{mso-style-type:export-only;
font-size:10.0pt;}
@page WordSection1
{size:8.5in 11.0in;
margin:1.0in 1.0in 1.0in 1.0in;}
div.WordSection1
{page:WordSection1;}
--></style></body></html>