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<meta http-equiv="Content-Type" content="text/html; charset=Windows-1252"></head><body style="word-wrap: break-word; -webkit-nbsp-mode: space; -webkit-line-break: after-white-space; color: rgb(0, 0, 0); font-size: 14px; font-family: Calibri, sans-serif; "><div><div><div><b><span style="font-size: 12pt; font-family: 'Times New Roman', serif; "><o:p> </o:p></span></b></div></div></div><span id="OLK_SRC_BODY_SECTION"><div xmlns:v="urn:schemas-microsoft-com:vml" xmlns:o="urn:schemas-microsoft-com:office:office" xmlns:w="urn:schemas-microsoft-com:office:word" xmlns:m="http://schemas.microsoft.com/office/2004/12/omml" xmlns="http://www.w3.org/TR/REC-html40"><div lang="EN-US" link="blue" vlink="purple"><div class="WordSection1"><p class="MsoNormal" align="center" style="mso-margin-top-alt:auto;margin-bottom:6.0pt;text-align:center;line-height:18.0pt"><a name="_Toc434675189"><b><span style="font-size: 14pt; font-family: 'Times New Roman', serif; color: black; ">MS THESIS DEFENSE STUDENT: </span></b></a><span style="font-size: 14pt; font-family: 'Times New Roman', serif; color: black; ">Ryan
McLay</span><span style="font-size: 14pt; font-family: 'Times New Roman', serif; color: black; "><o:p></o:p></span></p><p class="MsoNormal" align="center" style="mso-margin-top-alt:auto;margin-bottom:6.0pt;text-align:center;line-height:18.0pt"><b><span style="font-size: 14pt; font-family: 'Times New Roman', serif; color: black; ">DATE: </span></b><span style="font-size: 14pt; font-family: 'Times New Roman', serif; color: black; ">Monday,<b></b>May 2, 2016</span><span style="font-size: 14pt; font-family: 'Times New Roman', serif; color: black; "><o:p></o:p></span></p><p class="MsoNormal" align="center" style="mso-margin-top-alt:auto;margin-bottom:6.0pt;text-align:center;line-height:18.0pt"><b><span style="font-size: 14pt; font-family: 'Times New Roman', serif; color: black; ">TIME: </span></b><span style="font-size: 14pt; font-family: 'Times New Roman', serif; color: black; ">10:00 AM</span><span style="font-size: 14pt; font-family: 'Times New Roman', serif; color: black; "><o:p></o:p></span></p><p class="MsoNormal" align="center" style="mso-margin-top-alt:auto;margin-bottom:6.0pt;text-align:center;line-height:18.0pt"><b><span style="font-size: 14pt; font-family: 'Times New Roman', serif; color: black; ">PLACE: </span></b><span style="font-size: 14pt; font-family: 'Times New Roman', serif; color: black; ">Mechanical Engineering
Large Conference Room</span><span style="font-size: 14pt; font-family: 'Times New Roman', serif; color: black; "><o:p></o:p></span></p><p class="MsoNormal" align="center" style="mso-margin-top-alt:auto;margin-bottom:6.0pt;text-align:center;line-height:18.0pt"><b><span style="font-size: 14pt; font-family: 'Times New Roman', serif; color: black; ">DISSERTATION CHAIRS: </span></b><span style="font-size: 14pt; font-family: 'Times New Roman', serif; color: black; ">Dr. Jacinta
Conrad and Dr. Patrick Cirino</span><span style="font-size: 14pt; font-family: 'Times New Roman', serif; color: black; "><o:p></o:p></span></p><div style="margin-bottom:10.0pt"><div class="MsoNormal" align="center" style="margin-bottom:0in;margin-bottom:.0001pt;text-align:center"><b><span style="font-size: 14pt; line-height: 115%; font-family: 'Times New Roman', serif; color: black; "><hr size="2" width="100%" align="center"></span></b></div></div><p class="MsoNormal" align="center" style="text-align:center"><b><span style="font-size: 14pt; line-height: 115%; font-family: 'Times New Roman', serif; color: black; ">TITLE:<o:p></o:p></span></b></p><pre style="text-align:center"><b><span style="font-size: 14pt; font-family: 'Times New Roman', serif; ">Engineering <span style="color:black">Alkane-Inducible Fimbriation in <i>Escherichia coli</i></span><o:p></o:p></span></b></pre><p class="MsoNormal" align="center" style="text-align:center"><b><span style="font-size: 12pt; line-height: 115%; font-family: 'Times New Roman', serif; "><o:p> </o:p></span></b></p><p class="MsoNormal" style="margin-bottom:0in;margin-bottom:.0001pt;text-align:justify;line-height:200%"><span style="font-size: 12pt; line-height: 200%; font-family: 'Times New Roman', serif; color: black; ">Fimbriae are small, hair-like appendages arranged on many bacteria’s outer-membrane surfaces used for binding to biotic and abiotic surfaces to resist shear forces
and attach to nutrient sources. Fimbriae are important in the initial attachment of cells to a substrate as well as the subsequent irreversible attachment to a surface</span><span style="font-size: 12pt; line-height: 200%; font-family: 'Times New Roman', serif; ">.
<span style="color:black">In this investigation, <i>E. coli</i> was engineered to over-express fimbriae in response to the presence of IPTG and DCPK. The over-expression of fimbriae in
<i>E. coli</i> resulted in a pronounced response to the agglutination of mannose-containing yeast cells, causing them to sediment from solution at a much faster rate than wild-type or fimbrial deletion strains. Furthermore, the presence of fimbriae resulted
in a significantly higher partitioning of cells into hydrocarbon sources across a range of alkanes. Lastly, the presence of fimbriae was shown to be essential in the development of biofilms, where only strains expressing fimbriae resulted in biofilm formation.</span><o:p></o:p></span></p></div></div></div></span><style><!--
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