[CCoE Notice] SHARE WITH FACULTY: RFP - Single Cell RNA Sequencing Award from UH Sequencing and Gene Editing Core

[Knudsen, Rachel W]

The UH Sequencing and Gene Editing Core has a Request for Proposals that is open to UH investigators only.  Eligible engineering faculty are encouraged to apply.  The proposal submission deadline is Jan. 10, 2020. Please see attached and below for details.
Kathy

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REQUEST FOR PROPOSALS
Sequencing and Gene Editing Core - Single cell RNA Sequencing (scRNA-Seq) Award

BACKGROUND

Some of the most significant insights into the complex etiology and diversity of various diseases and dysfunctions have been discovered through genomic profiling and accelerated via next-generation sequencing.  These findings have been magnified by the ability to probe at single-cell resolution. The 10x Genomics single cell technology can analyze up to 10,000 single cells alongside Illumina’s next-generation sequencing platform.  The UH Sequencing and Gene Editing Core supports UH researchers by providing access to this single-cell gene expression technology, analysis, and integration.

This Single-Cell RNA Sequencing Award covers library preparation and bioinformatics support for up to two (2), two (2) sample pilot projects using the single cell gene expression sequencing application (https://www.10xgenomics.com/solutions/single-cell/) from 10x Genomics.


PURPOSE

The goals of this funding opportunity are three-fold:


(1)   support investigators’ use of scRNA-seq to acquire preliminary data for external funding, test feasibility of scRNA-seq as an approach, and/or validate data needed for publication;



(2)   encourage the use of scRNA-seq by investigators who are new to the technology; and


(3)   promote a collaborative environment between the UH Sequencing and Gene Editing Core and UH Researchers.

FUNDING

The UH Sequencing and Gene Editing Core will provide the following to the selected winners:

·         Project Consultation
·         Sample Quantification/QC
·         Single Cell capture and Library Preparation
·         Next-generation Sequencing
·         Bioinformatics Support
·         Invitation to present at the following UH Sequencing and Gene Editing Core seminar.

ELIGIBILITY

ONLY University of Houston investigators are eligible to apply. Those that attended the UH Sequencing and Gene Editing Core seminar (11/21/19) will receive additional consideration.  Laboratories can submit more than one (1) proposal; however, only one (1) proposal within a laboratory can be selected as a winner.

PROPOSAL REQUIREMENTS


·         Proposal abstract within a max of 300 words.

·         Abstract should include:

o    Background/Rationale

o    scRNA-seq Approach using 10X Genomics gene expression platform.

https://www.10xgenomics.com/solutions/single-cell/

o    scRNA-seq Impact

o    Overall Research Significance

·         Format:

o    US Letter size layout paper (8.5”x11”)

o    1-inch margins on all sides

o    Arial font size 11 pt.

o    1.5 line spacing

o    One (1) figure/table can be included within the proposal. The caption text must be legible (Arial; 8 pt. min.) and will not be counted towards 300-word max.

o    Applications that do not follow the formatting requirements will not be reviewed.

SUBMISSION REQUIREMENTS AND TIMELINE

Submit the completed application (.pdf only) to uhsequencing at gmail.com<mailto:uhsequencing at gmail.com>.

Submit document with the following Subject line and filename format:

“SingleCell_LastName_FirstName”

Ex)  SingleCell_Doe_John

Submission Opens:  Thursday, November 21, 2019

Proposal Submission Deadline:  Friday, January 10, 2020 5:00 PM (CST)

Winner(s) Announced by:  Monday, February 10, 2020

Start of Projects: latest 6 months after selection notification.

CONTACT INFORMATION

General questions related to the RFP/Submission should be directed to kholloway3 at uh.edu<mailto:kholloway3 at uh.edu> or 713-743-9291 (SERC 4008 office).

WEBSITE

http://seqnedit.nsm.uh.edu/

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