[CCoE Notice] Ph.D. Dissertation Announcement

[Khator, Suresh]

PhD Dissertation Announcement
Laser Multi-Spectral Diffuse Scatter Confocal Microscopy
Bian Hu

Laser scanning confocal microscopy (LSCM) is widely used in biological science. However, in most applications, the laser is only used as a monochromatic excitation source for fluorescence. Many advantageous aspects of laser technology and the scattering property are not fully utilized. Also conventional LSCM measures only on-axis scattered light, neglecting the large angle scattering which contains information of target that would not be easily obtained by onaxis detection.

The objective of this work is to develop a novel optical microscopy technique based on two key features: laser multi-spectral confocal imaging and large angle azimuthal scatter diffuse
imaging. Both features together give a combined spatial-spectral discrimination capability that can enhance contrast and spatial resolution not available from current optical microscopy. The
goal is to contribute a new tool for biomedical, analytical, sensing and metrological applications. This work describes the principle of laser multi-spectral diffuse scatter confocal microscopy.
Theoretical calculation and image simulation based on Finite Difference Time Domain (FDTD) are explored and provides theoretical basis and guide for experiments. Simulated confocal
images and large angle scatter diffuse images of several targets are obtained and discussed. To the best of our knowledge, it is never done by any other researchers. The microscopic
imaging system is based on a system architecture design configured similarly to an optical wireless network that allows multiple communication connections simultaneously among any
number of transmitters and receivers. The whole imaging system includes control program, optical components layout etc is optimized. Confocal images and large angle scatter diffuse
images are obtained and demonstrate high dynamic range and high image contrast of the imaging system. The experimental results are promising with capability to uncover and interpret
features that are not easily achieved by conventional confocal microscopic method. It proves that Laser Multi-Spectral Diffuse Scatter Confocal Microscopy is novel technology that can
obtain full information about targets and widely used for biomedical, analytical, sensing and metrological applications.

Committee Chair: Dr.Han Le         Place: E313              Date: Nov 12,2010            Time:1:00pm-3:00pm

Committee Members: Dr. Ji Chen, Dr. Jiming Bao, Dr. Len Trombetta, Dr. Loannis A Kakadiaris
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