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<p class="MsoNormal"><span style="font-size:13.5pt;font-family:"Arial",sans-serif"><a href="https://www.chee.uh.edu" target="_blank"><span style="color:#C8102E;text-decoration:none"><img border="0" width="600" height="165" style="width:6.2541in;height:1.7208in" id="_x0000_i1025" src="https://www.egr.uh.edu/sites/www.egr.uh.edu/files/enews/2022/images/sa_header.png" alt="William A. Brookshire Department of Chemical and Biomolecular Engineering Seminar Series"></span></a><o:p></o:p></span></p>
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<b><span lang="EN-GB" style="font-size:18.0pt;font-family:"Times New Roman",serif">Understanding and Mitigating Host-Part Incompatibilities During Microbial Engineering<o:p></o:p></span></b></p>
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<b><span style="font-size:18.0pt">Nikhil Nair</span></b><span style="font-size:16.0pt;font-family:"Arial",sans-serif"><br>
A</span><span style="font-size:16.0pt">ssociate Professor</span><span style="font-size:16.0pt;font-family:"Arial",sans-serif"><br>
T</span><span style="font-size:16.0pt">ufts University</span><span style="font-size:16.0pt;font-family:"Arial",sans-serif"><o:p></o:p></span></p>
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<strong><span style="font-size:12.0pt;font-family:"Arial",sans-serif">Friday, Sept 13 | 10:30a Central</span></strong><b><span style="font-size:12.0pt;font-family:"Arial",sans-serif"><br>
</span></b>Engineering 1, room L2D2<span style="font-size:12.0pt;font-family:"Arial",sans-serif"><o:p></o:p></span></p>
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<strong><span style="font-size:12.0pt;font-family:"Arial",sans-serif;color:#C8102E">LECTURE ABSTRACT</span></strong><span style="font-size:12.0pt;font-family:"Arial",sans-serif;color:#C8102E"><o:p></o:p></span></p>
<p class="MsoNormal"><span style="font-family:"Times New Roman",serif">One of our major goals is to elucidate and highlight the unexpected outcomes that result from modifying living systems and formalize them under the umbrella of “incompatibilities”. For example,
when multiple recombinant proteins are co-expressed in bacteria like <i>E. coli,</i> the cellular growth rate reduces, due to the
<i>burden</i> of protein expression. However, the same system can be considered as an
<i>incompatibility</i> between the resources used for protein synthesis and the bacterial host’s intrinsic resource demands for growth. Similarly, when a recombinant enzyme is expressed in a recombinant host, its off-target activity on host metabolites can
result in re-distribution of fluxes through a number of host metabolic pathways. While such activity is frequently filed under
<i>promiscuous</i> <i>enzymatic activity</i>, the same can be considered an <i>incompatibility</i> between the enzyme and the host’s metabolic network. We have spent significant effort in systematically exploring the origin these numerous host-part incompatibilities
(where, the added component, like recombinant protein, is referred to as a biological “part”) in efforts to explain previously inexplicable experimental observations. By understanding the origins of incompatibilities, our work has revealed fundamental insights
into cellular physiology and enabled development of more robust and efficient engineered biological systems.
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<strong><span style="font-size:12.0pt;font-family:"Arial",sans-serif;color:#C8102E">SPEAKER BIOSKETCH</span></strong><span style="font-size:12.0pt;font-family:"Arial",sans-serif;color:#C8102E"><o:p></o:p></span></p>
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Nair (naa-year) received his B.S. in Chemical and Biomolecular Engineering from Cornell University (Ithaca, NY) in 2003. While at Cornell, he was a founding member and lead guitarist of the not-so-well-known progressive metal band called “Rubicon”. After graduation
in 2003, he had a brief stint at Bristol Myers Squibb where he worked as a manufacturing research scientist in biotechnology purification development. He then went on to receive his M.S. and Ph.D. in Chemical and Biomolecular Engineering from the University
of Illinois, Urbana-Champaign under the guidance of Prof. Huimin Zhao. He joined Tufts in 2013 after completing a 3-year postdoctoral fellowship in Microbiology and Immunobiology at the Harvard Medical School in Prof. Ann Hochschild’s lab. He was promoted
to Associate Professor with tenure in 2020. He is a recipient of the 2016 NIH Director’s New Innovator Award. The Nair Synthetic Biology & Systems Bioengineering Lab focuses on two major areas of research – 1) biosynthesis of renewable fuels and chemicals
from sustainable feedstocks, and 2) engineering proteins and microbes to improve human health. In his spare time, which is increasingly rare, he likes to play guitar, golf, and video games and watch trashy TV shows like
<i>90 Day Fiancé</i> and <i>Sister Wives</i>. His long-term plans include starting several companies based on lab-developed technologies and eventually resurrecting “Rubicon” once his young sons are old enough to master their instruments (Kiran: guitar; Liam:
keyboards).<o:p></o:p></span></p>
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<em><span style="font-size:8.5pt;font-family:"Arial",sans-serif">This is an official message sent by the William A. Brookshire Department of Chemical & Biomolecular Engineering.</span></em><span style="font-size:8.5pt;font-family:"Arial",sans-serif"><o:p></o:p></span></p>
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<p class="MsoNormal"><span style="font-size:13.5pt;font-family:"Arial",sans-serif"><a href="https://www.chee.uh.edu" target="_blank"><span style="color:#C8102E;text-decoration:none"><img border="0" width="600" height="165" style="width:6.2541in;height:1.7166in" id="_x0000_i1027" src="https://www.egr.uh.edu/sites/www.egr.uh.edu/files/enews/2022/images/sa_footer.png" alt="William A. Brookshire Department of Chemical and Biomolecular Engineering"></span></a><o:p></o:p></span></p>
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