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<p class="xmsonormal" align="center" style="text-align:center"><a href="http://www.chee.uh.edu/" title="http://www.chee.uh.edu/"><span style="text-decoration:none"><img border="0" width="1250" height="344" style="width:13.0208in;height:3.5833in" id="x__x005f_x0000_i1026" src="cid:eadf286b-edc0-49de-986e-326f92ba5213"></span></a><o:p></o:p></p>
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<p align="center" style="text-align:center;background:white"><b><span style="font-size:18.0pt;font-family:&quot;Arial&quot;,sans-serif;color:#201F1E">Biomaterial Infection and Innate Immunity</span></b><span style="color:#201F1E"><o:p></o:p></span></p>
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<span style="font-size:10.5pt;font-family:&quot;Arial&quot;,sans-serif;color:#333333">&nbsp;</span><span style="color:#201F1E"><o:p></o:p></span></p>
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<span style="font-size:10.5pt;font-family:&quot;Arial&quot;,sans-serif;color:#333333">&nbsp;</span><span style="color:#201F1E"><o:p></o:p></span></p>
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<span style="font-size:10.5pt;font-family:&quot;Arial&quot;,sans-serif;color:#333333">&nbsp;</span><span style="color:#201F1E"><o:p></o:p></span></p>
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<b><u><span style="font-size:14.0pt;font-family:&quot;Arial&quot;,sans-serif;color:#201F1E">Philip S. Stewart</span></u></b><span style="color:#201F1E"><o:p></o:p></span></p>
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<span style="font-size:10.5pt;font-family:&quot;Arial&quot;,sans-serif;color:#201F1E">Regents Professor</span><span style="color:#201F1E"><o:p></o:p></span></p>
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<span style="font-size:10.5pt;font-family:&quot;Arial&quot;,sans-serif;color:#201F1E">Montana State University</span><span style="color:#201F1E"><o:p></o:p></span></p>
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<span style="font-size:10.5pt;font-family:&quot;Arial&quot;,sans-serif;color:#333333">&nbsp;</span><span style="color:#201F1E"><o:p></o:p></span></p>
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<b><span style="font-size:14.0pt;font-family:&quot;Arial&quot;,sans-serif;color:#201F1E">October 16 2020&nbsp;</span></b><b><span style="font-size:14.0pt;font-family:&quot;Arial&quot;,sans-serif;color:#333333">|&nbsp;</span></b><b><span style="font-size:14.0pt;font-family:&quot;Arial&quot;,sans-serif;color:#201F1E">10:30am
 Central time</span></b><span style="color:#201F1E"><o:p></o:p></span></p>
<p align="center" style="text-align:center"><span style="font-family:&quot;Calibri&quot;,sans-serif">Join Zoom Meeting</span>
<br>
<span style="font-family:&quot;Calibri&quot;,sans-serif"><a href="https://urldefense.com/v3/__https://uofh.zoom.us/j/97578286068__;!!LkSTlj0I!UI_n8S15XYep0DyPhui_-zRVKBO-Z_59mrALK5bhdIEf5ktjdAgW2QGdM9lBZ5qcVBLk$">https://uofh.zoom.us/j/97578286068</a></span><o:p></o:p></p>
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<span style="font-family:&quot;Calibri&quot;,sans-serif">Meeting ID: 975 7828 6068</span><span style="font-size:10.5pt;font-family:&quot;Arial&quot;,sans-serif;color:#333333">&nbsp;</span><span style="color:#201F1E"><o:p></o:p></span></p>
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<span style="font-size:10.5pt;font-family:&quot;Arial&quot;,sans-serif;color:#333333">&nbsp;</span><span style="color:#201F1E"><o:p></o:p></span></p>
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<b><u><span style="font-family:&quot;Arial&quot;,sans-serif;color:#C82613">LECTURE ABSTRACT</span></u></b><span style="color:#201F1E"><o:p></o:p></span></p>
<p class="xmsonormal" style="background:white;text-align:start"><span style="color:#201F1E">This presentation will develop the hypothesis that early events in the innate immune response to a microbially contaminated implant determine whether a biofilm forms
 and a persistent infection occurs.&nbsp; A corollary of this hypothesis is that innate immunity can be locally guided or boosted to reduce infections on indwelling medical devices.&nbsp; To gain insight into the important phenomena in the interaction between a nascent
 biofilm and phagocytes, two systems were used to directly and dynamically image the activity of neutrophils against small aggregates of surface-attached&nbsp;<i>Staphylococcus aureus</i>.&nbsp;&nbsp;<i>In vitro</i>, human neutrophils were stained red and added to a serum-coated
 glass cover slip with pre-attached GFP-expressing&nbsp;<i>S. aureus</i>.&nbsp;&nbsp;<i>In vivo</i>, multiphoton confocal intravital imaging was accomplished in a mouse model in which a young&nbsp;<i>S. aureus</i>&nbsp;biofilm attached to a glass coverslip was interfaced to the bottom
 of the dermis in a mouse skin flap model.&nbsp; Bacteria were imaged via GFP, neutrophils by tdTomato (CatchupIVM-red mice), collagen by second harmonic generation, and vasculature by injected quantum dots.&nbsp; Neutrophil speed, migration, discovery of bacteria, and
 killing of bacteria were tracked and quantified through image analysis.&nbsp; Collectively these observations suggest that: 1) the timescale for arrival of neutrophils at the biofilm-affected material surface in this animal model is variable with a median longer
 than three hours, 2) bacteria continue to grow prior to neutrophil arrival both&nbsp;<i>in vitro</i>&nbsp;and&nbsp;<i>in vivo</i>, and 3) formation of bacterial aggregates makes it more difficult for neutrophils to clear the nascent biofilm.<o:p></o:p></span></p>
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<span style="font-size:10.5pt;font-family:&quot;Arial&quot;,sans-serif;color:#333333">&nbsp;</span><span style="color:#201F1E"><o:p></o:p></span></p>
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<b><u><span style="font-family:&quot;Arial&quot;,sans-serif;color:#C82613">SPEAKER BIOSKETCH</span></u></b><span style="color:#201F1E"><o:p></o:p></span></p>
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<span style="font-size:10.5pt;font-family:&quot;Arial&quot;,sans-serif;color:#333333">&nbsp;</span><span style="color:#201F1E"><o:p></o:p></span></p>
<p style="background:white;text-align:start"><span style="color:#201F1E">Dr. Stewart is a Regents Professor of Chemical and Biological Engineering at Montana State University.&nbsp; Regents Professor is the most prestigious designation attainable by a professor
 in the Montana University System.&nbsp; He&nbsp;received his B.S. (1982) from Rice University, and M.S. (1985) and Ph.D. (1988) degrees from Stanford University, all in chemical engineering.&nbsp; Dr. Stewart has been integrally involved with the Center for Biofilm Engineering
 since his arrival on the Montana State campus, serving as director from 2005-2015.&nbsp; Dr. Stewart&#8217;s research focuses on the control of detrimental microbial biofilms, mechanisms of antimicrobial tolerance in biofilms, and interactions between biofilms and innate
 immune cells in the context of biofilm infection.&nbsp;<o:p></o:p></span></p>
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<span style="font-size:10.5pt;font-family:&quot;Arial&quot;,sans-serif;color:#333333">&nbsp;</span><span style="color:#201F1E"><o:p></o:p></span></p>
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<span style="font-size:10.5pt;font-family:&quot;Arial&quot;,sans-serif;color:#333333">&nbsp;</span><span style="color:#201F1E"><o:p></o:p></span></p>
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<span style="font-size:10.5pt;font-family:&quot;Arial&quot;,sans-serif;color:#333333">&nbsp;</span><span style="color:#201F1E"><o:p></o:p></span></p>
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<i><span style="font-size:8.0pt;font-family:&quot;Arial&quot;,sans-serif;color:black;background:white">This is an official message sent by the William A. Brookshire Department of Chemical &amp; Biomolecular Engineering.</span></i><span style="color:#201F1E"><o:p></o:p></span></p>
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<p class="xmsonormal" align="center" style="text-align:center"><a href="https://www.egr.uh.edu" target="_blank"><span style="text-decoration:none"><img border="0" width="1250" height="344" style="width:13.0208in;height:3.5833in" id="x__x005f_x0000_i1028" src="cid:e72811c1-2aba-49fb-bcb2-64eba62f3a54"></span><br>
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<p align="center" style="text-align:center;line-height:12.0pt"><span style="font-size:8.5pt;font-family:&quot;Arial&quot;,sans-serif;color:#333333">UH Cullen College of Engineering<br>
E421 Engineering Bldg 2, Houston, TX 77204-4007</span><o:p></o:p></p>
<p align="center" style="text-align:center;line-height:12.0pt"><span style="font-size:8.5pt;font-family:&quot;Arial&quot;,sans-serif;color:#333333">713-743-4200</span><o:p></o:p></p>
<p align="center" style="text-align:center;line-height:12.0pt"><span style="font-size:8.5pt;font-family:&quot;Arial&quot;,sans-serif;color:#333333">The University of Houston is an EEO/AA institution.</span><o:p></o:p></p>
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