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<p class="xmsonormal" align="center" style="text-align:center"><a href="http://www.chee.uh.edu/" title="http://www.chee.uh.edu/"><span style="text-decoration:none"><img border="0" width="1250" height="344" style="width:13.0208in;height:3.5833in" id="x__x005f_x0000_i1026" src="cid:eadf286b-edc0-49de-986e-326f92ba5213"></span></a><o:p></o:p></p>
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<p align="center" style="text-align:center;background:white"><b><span style="font-size:18.0pt;font-family:"Arial",sans-serif;color:#201F1E">Biomaterial Infection and Innate Immunity</span></b><span style="color:#201F1E"><o:p></o:p></span></p>
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<span style="font-size:10.5pt;font-family:"Arial",sans-serif;color:#333333"> </span><span style="color:#201F1E"><o:p></o:p></span></p>
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<span style="font-size:10.5pt;font-family:"Arial",sans-serif;color:#333333"> </span><span style="color:#201F1E"><o:p></o:p></span></p>
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<span style="font-size:10.5pt;font-family:"Arial",sans-serif;color:#333333"> </span><span style="color:#201F1E"><o:p></o:p></span></p>
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<b><u><span style="font-size:14.0pt;font-family:"Arial",sans-serif;color:#201F1E">Philip S. Stewart</span></u></b><span style="color:#201F1E"><o:p></o:p></span></p>
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<span style="font-size:10.5pt;font-family:"Arial",sans-serif;color:#201F1E">Regents Professor</span><span style="color:#201F1E"><o:p></o:p></span></p>
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<span style="font-size:10.5pt;font-family:"Arial",sans-serif;color:#201F1E">Montana State University</span><span style="color:#201F1E"><o:p></o:p></span></p>
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<span style="font-size:10.5pt;font-family:"Arial",sans-serif;color:#333333"> </span><span style="color:#201F1E"><o:p></o:p></span></p>
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<b><span style="font-size:14.0pt;font-family:"Arial",sans-serif;color:#201F1E">October 16 2020 </span></b><b><span style="font-size:14.0pt;font-family:"Arial",sans-serif;color:#333333">| </span></b><b><span style="font-size:14.0pt;font-family:"Arial",sans-serif;color:#201F1E">10:30am
Central time</span></b><span style="color:#201F1E"><o:p></o:p></span></p>
<p align="center" style="text-align:center"><span style="font-family:"Calibri",sans-serif">Join Zoom Meeting</span>
<br>
<span style="font-family:"Calibri",sans-serif"><a href="https://urldefense.com/v3/__https://uofh.zoom.us/j/97578286068__;!!LkSTlj0I!UI_n8S15XYep0DyPhui_-zRVKBO-Z_59mrALK5bhdIEf5ktjdAgW2QGdM9lBZ5qcVBLk$">https://uofh.zoom.us/j/97578286068</a></span><o:p></o:p></p>
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<span style="font-family:"Calibri",sans-serif">Meeting ID: 975 7828 6068</span><span style="font-size:10.5pt;font-family:"Arial",sans-serif;color:#333333"> </span><span style="color:#201F1E"><o:p></o:p></span></p>
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<span style="font-size:10.5pt;font-family:"Arial",sans-serif;color:#333333"> </span><span style="color:#201F1E"><o:p></o:p></span></p>
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<b><u><span style="font-family:"Arial",sans-serif;color:#C82613">LECTURE ABSTRACT</span></u></b><span style="color:#201F1E"><o:p></o:p></span></p>
<p class="xmsonormal" style="background:white;text-align:start"><span style="color:#201F1E">This presentation will develop the hypothesis that early events in the innate immune response to a microbially contaminated implant determine whether a biofilm forms
and a persistent infection occurs. A corollary of this hypothesis is that innate immunity can be locally guided or boosted to reduce infections on indwelling medical devices. To gain insight into the important phenomena in the interaction between a nascent
biofilm and phagocytes, two systems were used to directly and dynamically image the activity of neutrophils against small aggregates of surface-attached <i>Staphylococcus aureus</i>. <i>In vitro</i>, human neutrophils were stained red and added to a serum-coated
glass cover slip with pre-attached GFP-expressing <i>S. aureus</i>. <i>In vivo</i>, multiphoton confocal intravital imaging was accomplished in a mouse model in which a young <i>S. aureus</i> biofilm attached to a glass coverslip was interfaced to the bottom
of the dermis in a mouse skin flap model. Bacteria were imaged via GFP, neutrophils by tdTomato (CatchupIVM-red mice), collagen by second harmonic generation, and vasculature by injected quantum dots. Neutrophil speed, migration, discovery of bacteria, and
killing of bacteria were tracked and quantified through image analysis. Collectively these observations suggest that: 1) the timescale for arrival of neutrophils at the biofilm-affected material surface in this animal model is variable with a median longer
than three hours, 2) bacteria continue to grow prior to neutrophil arrival both <i>in vitro</i> and <i>in vivo</i>, and 3) formation of bacterial aggregates makes it more difficult for neutrophils to clear the nascent biofilm.<o:p></o:p></span></p>
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<span style="font-size:10.5pt;font-family:"Arial",sans-serif;color:#333333"> </span><span style="color:#201F1E"><o:p></o:p></span></p>
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<span style="font-size:10.5pt;font-family:"Arial",sans-serif;color:#333333"> </span><span style="color:#201F1E"><o:p></o:p></span></p>
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<b><u><span style="font-family:"Arial",sans-serif;color:#C82613">SPEAKER BIOSKETCH</span></u></b><span style="color:#201F1E"><o:p></o:p></span></p>
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<span style="font-size:10.5pt;font-family:"Arial",sans-serif;color:#333333"> </span><span style="color:#201F1E"><o:p></o:p></span></p>
<p style="background:white;text-align:start"><span style="color:#201F1E">Dr. Stewart is a Regents Professor of Chemical and Biological Engineering at Montana State University. Regents Professor is the most prestigious designation attainable by a professor
in the Montana University System. He received his B.S. (1982) from Rice University, and M.S. (1985) and Ph.D. (1988) degrees from Stanford University, all in chemical engineering. Dr. Stewart has been integrally involved with the Center for Biofilm Engineering
since his arrival on the Montana State campus, serving as director from 2005-2015. Dr. Stewart’s research focuses on the control of detrimental microbial biofilms, mechanisms of antimicrobial tolerance in biofilms, and interactions between biofilms and innate
immune cells in the context of biofilm infection. <o:p></o:p></span></p>
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<span style="font-size:10.5pt;font-family:"Arial",sans-serif;color:#333333"> </span><span style="color:#201F1E"><o:p></o:p></span></p>
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<span style="font-size:10.5pt;font-family:"Arial",sans-serif;color:#333333"> </span><span style="color:#201F1E"><o:p></o:p></span></p>
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<i><span style="font-size:8.0pt;font-family:"Arial",sans-serif;color:black;background:white">This is an official message sent by the William A. Brookshire Department of Chemical & Biomolecular Engineering.</span></i><span style="color:#201F1E"><o:p></o:p></span></p>
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<p align="center" style="text-align:center;line-height:12.0pt"><span style="font-size:8.5pt;font-family:"Arial",sans-serif;color:black"><a href="https://www.egr.uh.edu/" target="_blank"><span style="color:#C8102E">EGR HOME</span></a> | <a href="https://www.egr.uh.edu/news/" target="_blank"><span style="color:#C8102E">EGR
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<p align="center" style="text-align:center;line-height:12.0pt"><span style="font-size:8.5pt;font-family:"Arial",sans-serif;color:#333333">UH Cullen College of Engineering<br>
E421 Engineering Bldg 2, Houston, TX 77204-4007</span><o:p></o:p></p>
<p align="center" style="text-align:center;line-height:12.0pt"><span style="font-size:8.5pt;font-family:"Arial",sans-serif;color:#333333">713-743-4200</span><o:p></o:p></p>
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